TRIAP1, Recombinant, Human, aa1-76, His-Tag (TP53-Regulated Inhibitor of Apoptosis 1)
TP53-regulated inhibitor of apoptosis 1, also known as TRIAP1, contains a p53-binding site within its second exon and the reduction of expression by small interfering RNA enhanced apoptosis, whereas overexpression protected cells from apoptosis caused by DNA damage. TRIAP1 is induced significantly when cells have a low level of genotoxic stresses, but not when DNA damage is severe. TRIAP1 can modulate apoptotic pathways through interaction with Hsp70 that probably inhibits activity of apoptosis protease activating factor-1.
Source
Recombinant protein corresponding to aa1-76 from human TRIAP1, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~11.2kD (99aa), confirmed by MALDI-TOF
Applications
Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
