Wnt3a, Recombinant, Human (Protein Wnt-3a, Wingless-Type MMTV Integration Site Family, Member 3A, WNT3A)
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96% amino acid identity to mouse Wnt3a protein.
Source
Recombinant protein corresponding to aa19-352 of human Wnt3a, CHO cell line-derived.
Biological Activity
Wnt3a activity has been measured using TCF-based Wnt reporter stable cell lines (HEK293 and NIH3T3). 4ng/ml of Wnt3a generate fourteen- to twenty-fold increase of luciferase activity compared to control (buffer without Wnt3a).
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, CHO cells
Form
Supplied as a liquid in Tris-HCl, pH 7.2-7.4, 0.5% CHAPS, 0.1% BSA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
