361506
Grade
Affinity PurifiedSpecificity
Recognizes the epitope sequence, EQKLISEEDL, located in aa410-419 of human c-myc protein.Applications
IF IHC IP WBEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not Freezec-Myc Epitope Tag (Proto-oncogene c-Myc, Transcription Factor p64, Class E Basic Helix-loop-helix Protein 39, BHLHE39, Cmyc) (Chimeric)
Recombinant monoclonal antibody to C-myc Epitope Tag with variable regions (i.e., specificity) from the hybridoma 9E10. Manufactured using a proprietary recombinant platform technology. This is a chimeric human antibody, based on the original mouse IgG1 format.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen and paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
