Technical Data

377686
Grade
Purified
Accession Number
NP_001101
Molecular Weight
51.64057
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
ADAM10, Recombinant, Human, aa214-672, His-Tag (Disintegrin and Metalloproteinase Domain-containing Protein 10, AD10, AD18, CD156c, HsT18717, Kuz, MADM, RAK)

ADAM10, as known as disintegrin and metalloproteinase domain-containing protein 10 isoform 1, is a member of the ADAM family that contains a disintegrin and metallogrotease-like domain. This protein is a sheddase, and has a broad specificity for peptide hydrolysis reactions. Also, it cleaves ephrin, within the ephrin/eph complex, formed between two cell surfaces. When ephrin is freed from the opposing cell, the entire ephrin/eph complex is endocytosed. In neurons, ADAM10 is the most important enzyme with alpha-secretase activity for proteolytic processing of the amyloid precursor protein.

Source
Recombinant protein corresponding to aa214-672 from human ADAM10, fused to His-tag at C-terminal, expressed in insect cell.
Molecular Weight
~51.6kD (467aa), 50-70kD (SDS-PAGE under reducing conditions.)
Endotoxin
<1EU/1ug (determined by LAL method)
AA Sequence
TTSAEKNTCQ LYIQTDHLFF KYYGTREAVI AQISSHVKAI DTIYQTTDFS GIRNISFMVK RIRINTTADE KDPTNPFRFP NIGVEKFLEL NSEQNHDDYC LAYVFTDRDF DDGVLGLAWV GAPSGSSGGI CEKSKLYSDG KKKSLNTGII TVQNYGSHVP PKVSHITFAH EVGHNFGSPH DSGTECTPGE SKNLGQKENG NYIMYARATS GDKLNNNKFS LCSIRNISQV LEKKRNNCFV ESGQPICGNG MVEQGEECDC GYSDQCKDEC CFDANQPEGR KCKLKPGKQC SPSQGPCCTA QCAFKSKSEK CRDDSDCARE GICNGFTALC PASDPKPNFT DCNRHTQVCI NGQCAGSICE KYGLEECTCA SSDGKDDKEL CHVCCMKKMD PSTCASTGSV QWSRHFSGRT ITLQPGSPCN DFRGYCDVFM RCRLVDADGP LARLKKAIFS PELYENIAEL EHHHHHH
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, Baculovirus
Purity
~85% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Park GB., et al, (2015) Invest. Ophthalmol. Vis. Sci. 56:5162-5173.|2. Zingoni A., et al, (2015) J. Immunol. 195:736-748.
USBio References
No references available
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