Technical Data

377992
Grade
Highly Purified
Accession Number
NP_000356
Molecular Weight
28.8
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
TPI1, Recombinant, Human, aa1-249, His-Tag (Triosephosphate Isomerase, TPI)

TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Source
Recombinant protein corresponding to aa1-249 from human TPI1 protein, fused to His-tag at N-terminal, expressed in E. coli.
Molecular Weight
~28.8kD (269aa), confirmed by MALDI-TOF
Endotoxin
<1EU/1ug (determined by LAL method)
Biological Activity
Specific activity is >3000units/mg, in which one unit will convert 1umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate/minute at pH 7.5 at 25°C.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.5, 1mM DTT, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Maquat L.E., et al. (1985) J. Biol. Chem. 260:3748-3753.|2. Rodríguez-Almazán C, et al.(2008) J Biol Chem. 22;283(34):23254-63.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net