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544472 Spike S, Recombinant, SARS-CoV-2, aa16-685, Fc Fusion, Avi-tag (SARS-CoV-2) CAS:

Specifications
References
Grade
Purified
Swiss Prot
P0DTC2
Accession Number
QHD43416.1
Molecular Weight
104
Shipping Temp
Dry Ice
Storage Temp
-70°C
SARS-CoV s1, Coronavirus spike S1, Covid, Covid-19, Covid19, Coronavirus

Recombinant protein corresponding to aa16-685 from Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), fused at the C-terminus of the Fc portion of human IgG1, and a C-terminal Avi-tag, expressed in HEK293 cells.

Molecular Weight
~104kD. This protein runs at a higher MW by SDSPAGE due to glycosylation.
Assay Conditions
SARS-CoV-2 S1 and ACE2 binding assay was done using ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit. SARS-CoV-2 S1 protein and anti-human Fc-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively.
Applications
Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, HEK293 cells
Purity
≥90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 8mM Phosphate, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10. 2. Walls, A. C. et al., Cell. 2020 Mar; 180(2):281-292.
USBio References
No references available
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