ARRDC1 cDNA
ARRDC1 ARRDC2 (which exists as multiple alternatively spliced isoforms), ARRDC4 and ARRDC5 are arrestin domain-containing proteins that are encoded by genes which map to human chromosomes 9, 15 and 19.
Vector Description
This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Preparation before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Nucleotide Sequence
ATGGGGCGAGTGCAGCTCTTCGAGATCAGCCTGAGCCACGGCCGCGTCGTCTACAGCCCCGGGGAGCCGTTGGCTGGGACCGTGCGCGTGCGCCTGGGGGCACCGCTGCCGTTCCGAGCCATCCGGGTGACCTGCATAGGTTCCTGCGGGGTCTCCAACAAGGCTAATGACACAGCGTGGGTAGTGGAGGAGGGTTACTTCAACAGTTCCCTGTCGCTGGCAGACAAGGGGAGCCTGCCCGCTGGAGAGCACAGCTTCCCCTTCCAGTTCCTGCTTCCTGCCACTGCACCCACGTCCTTTGAGGGTCCTTTCGGGAAGATCGTGCACCAGGTGAGGGCCGCCATCCACACGCCACGGTTTTCCAAGGATCACAAGTGCAGCCTCGTGTTCTATATCTTGAGCCCCTTGAACCTGAACAGCATCCCAGACATTGAGCAACCCAACGTGGCCTCTGCCACCAAGAAGTTCTCCTACAAGCTGGTGAAGACGGGCAGCGTGGTCCTCACAGCCAGCACTGATCTCCGCGGCTATGTGGTGGGGCAGGCACTGCAGCTGCATGCCGACGTTGAGAACCAGTCAGGCAAGGACACCAGCCCTGTGGTGGCCAGTCTGCTGCAGAAAGTGTCCTATAAGGCCAAGCGCTGGATCCACGACGTACGGACCATTGCGGAGGTGGAGGGTGCGGGCGTCAAGGCCTGGCGGCGGGCGCAGTGGCACGAGCAGATCCTGGTGCCTGCCTTGCCCCAGTCGGCCCTGCCGGGCTGCAGCCTCATCCACATCGACTACTACTTACAGGTCTCTCTGAAGGCGCCGGAAGCTACTGTGACCCTCCCGGTCTTCATTGGCAATATTGCTGTGAACCATGCCCCAGTGAGCCCCCGGCCAGGCCTGGGGCTGCCTCCTGGGGCCCCACCCCTGGTGGTGCCTTCCGCACCACCCCAGGAGGAGGCTGAGGCTGAGGCTGCGGCTGGCGGCCCCCACTTCTTGGACCCCGTCTTCCTCTCCACCAAGAGCCATTCGCAGCGGCAGCCCCTGCTGGCCACCTTGAGTTCTGTGCCTGGTGCGCCGGAGCCCTGCCCTCAGGATGGCAGCCCTGCCTCACACCCGCTGCACCCTCCCTTGTGCATTTCAACAGGTGCCACTGTCCCCTACTTTGCAGAGGGCTCCGGGGGGCCAGTGCCCACTACCAGCACCTTGATTCTTCCTCCAGAGTACAGTTCTTGGGGCTACCCCTATGAGGCCCCACCGTCTTATGAGCAGAGCTGCGGCGGCGTGGAACCCAGCCTGACCCCTGAGAGCTGA
Translation Sequence
MGRVQLFEIS LSHGRVVYSP GEPLAGTVRV RLGAPLPFRA IRVTCIGSCG VSNKANDTAW VVEEGYFNSS LSLADKGSLP AGEHSFPFQF LLPATAPTSF EGPFGKIVHQ VRAAIHTPRF SKDHKCSLVF YILSPLNLNS IPDIEQPNVA SATKKFSYKL VKTGSVVLTA STDLRGYVVG QALQLHADVE NQSGKDTSPV VASLLQKVSY KAKRWIHDVR TIAEVEGAGV KAWRRAQWHE QILVPALPQS ALPGCSLIHI DYYLQVSLKA PEATVTLPVF IGNIAVNHAP VSPRPGLGLP PGAPPLVVPS APPQEEAEAE AAAGGPHFLD PVFLSTKSHS QRQPLLATLS SVPGAPEPCP QDGSPASHPL HPPLCISTGA TVPYFAEGSG GPVPTTSTLI LPPEYSSWGY PYEAPPSYEQ SCGGVEPSLT PES
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
