Technical Data

A0760-40K1
Grade
Purified
Applications
E
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Actin, beta (Non-muscle), Control Peptide

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g- smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.

Source
Synthetic peptide corresponding to N-terminal 15aa of human beta-actin, non-muscle.
Applications
Suitable for use in Antibody Blocking and ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Synthetic peptide
Purity
Purified
Concentration
As reported
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ohmuri H (1995) Gene Accession # S38782; 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451; 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349; 4. North JA et al (1994) J. Cell Sci. 107, 437.
USBio References
No references available
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Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net