Technical Data

A0882-72
Grade
Purified
Accession Number
AAH36666
Molecular Weight
65.9
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
AK5, Recombinant, Human, aa1-562, His-Tag (Adenylate Kinase Isoenzyme 5, AK 5, ATP-AMP Transphosphorylase 5)

AK5, is a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain.

Recombinant corresponding to aa1-562 from human AK5, fused to His-tag at N-terminal expressed in E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMNTNDA KEYMARREIP QLFESLLNGL MCSKPEDPVE YLESCLQKVK ELGGCDKVKW DTFVSQEKKT LPPLNGGQSR RSFLRNVMPG NSNFPYRRYD RLPPIHQFSI ESDTDLSETA ELIEEYEVFD PTRPRPKIIL VIGGPGSGKG TQSLKIAERY GFQYISVGEL LRKKIHSTSS NRKWSLIAKI ITTGELAPQE TTITEIKQKL MQIPDEEGIV IDGFPRDVAQ ALSFEDQICT PDLVVFLACA NQRLKERLLK RAEQQGRPDD NVKATQRRLM NFKQNAAPLV KYFQEKGLIM TFDADRDEDE VFYDISMAVD NKLFPNKEAA AGSSDLDPSM ILDTGEIIDT GSDYEDQGDD QLNVFGEDTM GGFMEDLRKC KIIFIIGGPG SGKGTQCEKL VEKYGFTHLS TGELLREELA SESERSKLIR DIMERGDLVP SGIVLELLKE AMVASLGDTR GFLIDGYPRE VKQGEEFGRR IGDPQLVICM DCSADTMTNR LLQRSRSSLP VDDTTKTIAK RLEAYYRASI PVIAYYETKT QLHKINAEGT PEDVFLQLCT AIDSIF
Molecular Weight
~65.9kD
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥85% (SDS-PAGE)
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 1mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Van Rompay A.R., et al. (1999) Eur. J. Biochem. 261:509-517. 2. Solaroli N., et al. (2009) FEBS Lett. 583(17):2872-6.
USBio References
No references available
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