Agarose Low EEO (EP)
Agarose Low EEO Electrophoresis Grade is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Low EEO Agarose is recommended for a wide array of molecular biology procedures and offers enhanced resolution ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
See A1015 for our best grade with complete Functional and Molecular Biology Testing.
Applications
• Analytical DNA Gels ≥ 1000bp • Blotting Assays (Southern, northern) • Radial Immunodiffusion • Restriction Enzyme digestion products • For use with all buffer systems
Recommended Usage (0.5X TBE)
Concentration Molecular Weight 1% 500bp-10kb 1.2% 350bp-7kb 1.5% 250bp-2kb
Appearance
White, fine, homogeneous, free flowing powder
EEO (pH 8.4, Wieme Method)
0.09-0.13
Melting Point(1.5%)
86.5-89.5°C
Gel Point (1.5%)
34.5-37.5°C
Gel Strength (1.5%)
≥2500g/cm2
Absorbance (450nm)
As Reported
Quality Control
Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
• Background Fluorescence (TAE/TE, EtBr)
Functional and Molecular Biology Testing: To Pass Test • Comparative assay of different size DNA fragments • Southern Blot (125-23,000bp) • DNA Binding (TAE, EtBr) • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Storage and Stability
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
Form
White, homogeneous free flowing powder
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
