A1124-07D
Grade
Highly PurifiedSpecificity
A specific substrate for Protein Kinase B alpha (PKB/ AKT; Km = 5uM). This peptide is not phosphorylated by p70 S6 kinase or MAP kinase activated protein (MAPKAP) kinase-1.Applications
EEU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
4°C/-20°CBSA Free
YesPreservative Free
YesNotes
Preservative Free
BSA Free
Akt, Substrate Peptide (Rac PKa, PKBa)
AKT is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3 at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
Peptide Sequence (linear)
R-P-R-A-A-T-F (Arg-Pro-Arg-Ala-Ala-Thr-Phe)
Applications
Suitable for use as a substrate for Protein Kinase B alpha (PKB/AKT; Km = 5uM) Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
95+%, HPLC, Mass Spec
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
