Technical Data

A3000-03C
Grade
Purified
Applications
E
Molecular Weight
3
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Aquaporin 1, Control Peptide (AQP1, CHIP-28, Channel-forming Integral Protein)

Control peptide for Aquaporin 1, Chicken Anti-rat, serum, polyclonal antibody.

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein.
Source
Synthetic peptide corresponding to 19aa of rat AQP1 (protein accession #P29975)
Molecular Weight
~3kD
Applications
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 50-100ng control peptide/well Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Synthetic peptide
Purity
Purified
Concentration
~1mg/ml
Form
Supplied as a lyophilized pwder from PBS, pH7.5.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lanahan, A. et al (1992) MCB, 12:3919- 3929. 2. Deen, P. et al (1992) Biochem.Biophys Res Comm. 188:1267- 1273. 3. Moon, C. et al (1993) JBC. 268:15772-15778. 4. Patil, R. et al (1994) ) Biochem. Biophys Res Comm. 204:861-866. 5. Smith, B. and Agre, P. (1991) JBC 266:6407-6415.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net