L-Asparaginase (Amidohydrolase)
L-Asparaginase is an enzyme that depletes L-Asparagine an important nutrient for cancer cells resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E. coli, which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease.
L-Asparaginase produced from E. coli containing 303 amino acids and having a molecular mass of 31.73kD.
Unit Definition
One unit of enzyme catalyzes the hydrolysis of 10 nanomoles of dUTP to dUMP in 1 hour at 85ºC.
Activity
One unit of A3800 is defined as the amount of enzyme required to generate 1umol of ammonia per minute at pH 7.3 and 37ºC.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
≥96% by RP-HPLC and SDS-PAGE.
Form
Supplied as a lyophilized powder without additives.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
