Technical Data

A4500
CAS Number
1405-69-2
Grade
Molecular Biology Grade
Gene ID
396260
Molecular Weight
6668
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C
Avidin (Egg White, 68kD Glycoprotein)

Avidin (Biotin Binding Protein) is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding log constant of 15. Avidin is a tetrameric protein, composed of subunits of identical amino acid composition and sequence. Each monomer is glycosylated on Asn-17.

The high affinity constant of Avidin for Biotin has been widely used in molecular biology: affinity chromatography, molecular recognition and labelling, Enzyme Linked ImmunoSorbent Assay (ELISA), histochemistry and cytochemistry. An excellent review in the field has been written by Wilchek, M. and Bayer, E.A., eds. (1990), 'Avidin-Biotin Technology', Methods in Enzymology, Vol.184, Academic Press.
Molecular Weight
Monomer at 16.5kD. Dimer at 33kD and Tetramer at 66kD may also appear under incomplete denaturation.
Source
Egg white
Purity
Purified by chromatography.
Protein
≥0.90 mg protein/mg solid
Protein Purity (10mg)
Homogeneous by SDS
Form
Supplied as a lyophilized powder.
Activity
≥12u/mg
Unit Definition
One unit will bind 1ug of d-biotin at pH 8.9.
Water
≤ 5%
Storage and Stability
Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at 4°C. Reconstituted product is stable for 6 months at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Source
Egg white
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Berger, M., and Wood, H.: Purification of the Subunits of Transcarboxylase by Affinity Chromatography on Avidin-Sepharose, J. Biol. Chem., 250, 927 (1975).|Fraenkel-Conrat, H., Snell, N., and Ducay, E.: Avidin. I. Isolation and Characterization of the Protein and Nucleic Acid, Arch. Biochem. Biophys., 39, 80 (1952).|Green, N.: Avidin. 3. The Nature of the Biotin-Binding Site, Biochem. J., 89, 599 (1963a).|Green, N.: Purification of Avidin, in Methods in Enzymology, XVIII, (McCormick, D., and Wright, L., eds.), Academic Press, NY, 414 (1970a).|Green, N.: The Molecular Weight of Avidin, Biochem. J., 92, 16c (1964).|Green, N.: A Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin, Biochem. J., 94, 23c (1965).|Melamed, M., and Green, N.: Avidin. 2. Purification and Composition, Biochem. J., 89, 591 (1963).|Wei, R., and Wright, L.: Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin, Proc. Soc. Exp. Biol. Med., 117, 341 (1964).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net