5'-T C G^C G A-3' 3'-A G C^G C T-5'

Bacillus megaterium RFL68

10u/ul

One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.

10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

10mM Tris-HCl pH 7.5, 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.

1X buffer formulation - 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.

1X buffer formulation - 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM sodium chloride and 0.1mg/ml BSA.

May overlap - no effect

Never overlaps - no effect

Completely overlaps - blocked

5

2

0

1

0

1

0