C1205
CAS Number
9025-24-5Grade
Molecular Biology GradeMolecular Weight
34.5EU Commodity Code
38220090Shipping Temp
Dry IceStorage Temp
-20°CCarboxypeptidase B
EC=3.4.17.2; Porcine pancreas; Protaminase; Peptidyl-L-Lysine [L-Arginine] hydrolase
Carboxypeptidase B (CPB) catalyzes hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-teminal position in polypeptides. It occurs as a zymogen in the pancreas of most vertebrates.
Synonyms
EC=3.4.17.2; Porcine pancreas; Protaminase; Peptidyl-L-Lysine [L-Arginine] hydrolase
Appearance
Chromatographically purified and supplied as a solution in 0.1M sodium chloride.
Purity
Chromatographically purified
Activity
≥170 units/mg protein
Unit Definition
1 unit hydrolyzes 1 micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Folk, J. E., Piez, K. A., Carroll, W. R. and Gladner, J., J. Biol. Chem., 235:2272 (1960).USBio References
No references available