Cellulase CAS 9012-54-8

CAS Number

9012-54-8

Grade

Molecular Biology Grade

EU Commodity Code

38220090

Shipping Temp

Storage Temp

4°C

Onozuka R-10

Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes.

Source

Trichoderma viride

Synonyms

Onozuka R-10

CAS No

9012-54-8

EC Number

3.2.1.4

Unit Definition of Activity

One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).

Major Enzyme Activity

Cellulase: 1u/mg

Extraneous Enzyme Activities

Pectinase: 0.4u/mg Hemicellulase: 1.0u/mg alpha-Amylase: 0.6u/mg Protease: 0.01u/mg

Optimum pH range

4-5

Appearance

Light yellow powder

Solubility

Soluble in water (10mg/ml)

Loss on Drying

≤10%

Arsenic

≤3ug

Heavy Metals

≤40ug

Activity

≥10,000U/g

Total Viable Count

≤10,000cfu/g

Bacteria

≤10,000cfu/g

Fungi

<100cfu/g

Coliform

Negative

Stability and Storage

Lyophilized powder may be stored at 4°C. Stable for 6 months at 4°C.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Toxicity and Hazards

All products should be handled by qualified personnel only, trained in laboratory procedures.

References

1. Beldman, G., et al., Eur. J. Biochem. 146: 301-308 (1985). 2. Potrykus, J. & Shillito, R.D., Methods Enzymol. 118: 549-578 (1986). 3. Okada, G., Methods Enzymol. Vol. 160: 259-263 (1988). 4. Lendl & Bauer, Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff (1989).

USBio References

No references available

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C2610-11 Cellulase (Onozuka R-10), Trichoderma viride CAS: 9012-54-8

CAS Number

Grade

EU Commodity Code

Shipping Temp

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Onozuka R-10

References

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