C2610-11
CAS Number
9012-54-8Grade
Molecular Biology GradeEU Commodity Code
38220090Shipping Temp
RTStorage Temp
4°CCellulase (Onozuka R-10), Trichoderma viride
Onozuka R-10
Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes.
Source
Trichoderma viride
Unit Definition of Activity
One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Major Enzyme Activity
Cellulase: 1u/mg
Extraneous Enzyme Activities
Pectinase: 0.4u/mg Hemicellulase: 1.0u/mg alpha-Amylase: 0.6u/mg Protease: 0.01u/mg
Appearance
Light yellow powder
Solubility
Soluble in water (10mg/ml)
Total Viable Count
≤10,000cfu/g
Stability and Storage
Lyophilized powder may be stored at 4°C. Stable for 6 months at 4°C.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Beldman, G., et al., Eur. J. Biochem. 146: 301-308 (1985). 2. Potrykus, J. & Shillito, R.D., Methods Enzymol. 118: 549-578 (1986). 3. Okada, G., Methods Enzymol. Vol. 160: 259-263 (1988). 4. Lendl & Bauer, Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff (1989).USBio References
No references available