Connexin 37, 43, GAP27, Scrambled Peptides (37,43Cx-Gap27)
Gap junctions contain channels that allow the passage of ions and small molecules between adjacent cells. This intercellular communication has been implicated in the coordination of cellular responses to intracellular signaling molecules. Connexins are a family of integral membrane proteins that oligomerize to form intercellular channels that are clustered at gap junctions. Connexins share a common transmembrane (TM) topology. Each connexin is thought to contain 4 TM domains, with two extracellular and three cytoplasmic regions.
Connexin-mimetic peptides, i.e. peptides that are identical with a short aa sequence on the Cx subunit have recently emerged as tools to block Connexin hemi-channels (CxHcs) with little or no immediate effects on gap junctions. Gap 27 corresponds to aa sequence on the second extracellular loop of Cx43. The sequence comprises conserved domain SRPTEK which interfaces with the membrane. Gap27 inhibits gap junctional transfer of fluorescent dyes, electrical coupling and synchronized Ca2+ oscillations in smooth-muscle cells.
Source
Human/Mouse/Rat 37,43Cx-Gap27, aa201-211, R-E-K-I-I-T-S-F-I-P-T.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile DMF. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder. Reconstitute with sterile DMF.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
