C7915-10A
Grade
PurifiedApplications
E WBEU Commodity Code
38220090Shipping Temp
RTStorage Temp
RTCREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein) Blocking Peptide
Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3–5). CREB appears to play an important role in learning and memory in both flies and mice.
Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser133 of human CREB.
Corresponding antibody, C7915-10, detects endogenous levels of CREB only when phosphorylated at serine 133. Also detects the phosphorylated form of CREB-related protein ATF-1. Species Crossreactivity: Mouse and rat.
Applications
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder.
Important Note
This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gonzalez, G.A. and Montminy, M.R. (1989) Cell 59, 675–680. 2. Sheng, M. et al. (1991) Science 252, 1427–1430. 3. Xing, J. et al. (1996) Science 273, 959–963. 4. Tan, Y. et al. (1996) EMBO J. 15, 4629–4642. 5. Deak, M. et al. (1998) EMBO J. 17, 4426–4441. 6. Constantinescu, A. et al. (2002) J. Biol. Chem. 277, 18810–18816. 7. Drahushuk, K. et al. (2002) J. Neurosci. 22, 6560–6569. 8. Grewal, S.S. et al. (2000) J. Biol. Chem. 275, 34433–34441. 9. Ho, N. et al. (2000) J. Neurosci. 20, 6459–6472.|10. Hu, B.R. et al. (1999) Neurosci. 89, 437–452. 11. Huang, H. et al. (2001) J. Biol. Chem. 276, 38830–38836. 12. Liu, X. and Green, C.B. (2002) Mol. Cell. Biol. 22, 7501–7511. 13. Maronde, E. et al. (1999) J. Neurosci. 19, 3326–3336. 14. Reusch, J.E. et al. (2000) Mol. Cell. Biol. 20, 1008–1020.|15. Xing, J. et al. (1998) Mol. Cell. Biol. 18, 1946–1955.USBio References
No references available