• Random primer labeling • Sanger sequencing • cDNA Second Strand Synthesis • 3’ end-labeling, filling • Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol • Oligonucleotide-directed site-specific mutagenesis.

E. coli cells with a cloned fragment of the polA gene

~10u/ul

Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 0.1mM EDTA, 1mM DTT, 50% glycerol.

1u of DNA Polymerase I Klenow Fragment catalyzes the incorporation of 10nmoles of deoxynucleotides into polynucleotide fraction in 30 minutes at 37°C.

Supplied as a liquid in 500mM Tris-HCl, pH 8.0, 50mM magnesium chloride, 10mM DTT

-Inhibitors: metal chelators, nucleotide analogs PPi, Proprietray information (at high concentrations). -Inactivated by heating at 75°C for 10 min or by addition of EDTA.

No detectable degradation was observed after incubation of supercoiled plasmid DNA with DNA Polymerase I Klenow Fragment.

Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

E. coli cells with a cloned fragment of the polA gene

~10u/ul

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.