DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant CAS

Grade

Molecular Biology Grade

EU Commodity Code

30021019

Shipping Temp

Blue Ice

Storage Temp

-20°C

EC=2.7.7.7; E. coli|Supplied with Reaction Buffer

This is an N-terminal truncation of the E. coli DNA Polymerase I protein, at amino acid #323. This truncation results in the deletion of the 5' to 3' exonuclease domain, but leaves the polymerase function unaffected. The introduction of two additional mutations (D355A and E357A) results in the abolition of the 3' to 5' exonuclease activity.

Source

E. coli

Unit Definition

1u catalyzes the incorporation of 10nmoles of deoxynucleotides into polynucleotide fraction in 30 minutes at 37°C.

D3941-01A: DNA Polymerase I Klenow Fragment, Reaction Buffer, 10X

Supplied as a liquid in 200mM Tris, pH 7.5, 50mM MgCl2, 20mM DTT.

Storage and Stability

Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Source

Recombinant, E. coli

Concentration

As Reported

Form

Supplied as a liquid in 50mM KPO4, pH 7.0, 100mM KCl, 1mM DTT, 50% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Derbyshire, V., et al., Science 240: 199-201 (1988). 2. Feinberg, A.P. and B. Vogelstein, Anal. Biochem. 137: 266-267 (1984). 3. Sanger, F., et al., Proc. Natl. Acad. Sci. USA 74: 5463-5467 (1977). 4. Walker, G.T., PCR Methods Appl. 3: 1-6 (1993). 5. Clark, J.M., et al., J. Mol. Biol. 198: 123-127 (1987).|6. Gubler, U. (1987) S.L. Berger and A.R. Kimmel (Eds.), Methods in Enzymology, 152, pp. 330-335. San Diego: Academic Press. 7. Joseph Gilbert, Jeremy Hasseman, Robin Cline,Kathy Munoz, Jon Hnath Microbial Genomic DNA aminoallyl labeling for microarrays. S.O.P. from The Institute for Genomic Research (TIGR).

USBio References

No references available

Product	Size	List	Your Price	Qty	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

Product

Size

List

Your Price

Qty

Ext Price

Subtotal:

Total Coupon Savings:

()

Your cart is currently empty.

- Coupon Code

D3941-01 DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant CAS:

Grade

EU Commodity Code

Shipping Temp

Storage Temp

EC=2.7.7.7; E. coli|Supplied with Reaction Buffer

References

USBio References

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods