Dipeptidyl Peptidase 7, Recombinant, Human (DPP7)
The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.
Source
Recombinant human, DPP7, aa26-end, with C-terminal HIS tag, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity
1882pmol/min/ug
Assay conditions
10mM Tris-HCl, pH7.4, 10mM MgCl2, 1mM MnCl2, 20uM Ala-Pro-AMC, 10 min, RT
Applications
Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Source
Baculovirus infected Sf9 cells
Form
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 130mM NaCl, 0.05% Tween-20, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
