Technical Data

E9073-50
Grade
Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Baculovirus Expression Vector pAc-k-CH3

Baculovirus cassette vector pAc-k-CH3 for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.

Vector Size
7363bp
Resistance
Vector contains an ampicillin resistance marker gene
Applications
Cloning of heavy and light chain variable domain genes of scFv antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.
Cloning sites
Xhol and Nhel for heavy chain VH genes. Sacl and HindIII for light chain VL(k) genes.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified using a Plasmid Purification Kit.
Concentration
~0.5mg/ml
Form
Supplied as a liquid in TE buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Liang, M., et al., (2001) J. Immunol. Meth. 247:119-130. 2. Liang, M. & Dübel, S., Expresssion of antibody fragments using the Baculovirus system. In: Antibody Engineering, ed: Kontermann, R. and Dübel, S. Springer Verlag, Heidelberg (2001). 3. Barbas, C.F. 3rd, et al., (1991) PNAS USA 88:7978-7982. 4. Nesbit, M., et al., (1992) J. Immunol. Meth. 151:201-208 . 5. Poul, M.A., et al., (1995) Immunotechnology 1:189-196. 6. Poul, M.A., et al., (1995) Eur. J. Immunol. 25:2005-2009. 7. Liang, M., et al., (1997) Virology 235:252-260. 8. Liang, M. & Dübel, S., Expression of antibody fragments using the Baculovirus system. In: Antibody Engineering, ed: Kontermann, R. & Dübel, S., Springer Verlag, Heidelberg (2001). 9. Liang, M., et al., (2001) J. Immunol. Meth. 247:119-130.
USBio References
No references available
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