Technical Data

F5950
Grade
Affinity Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
FOPflash (Mutant Tcf Binding Sites) (TK-luciferase Reporter) Negative Control

Transfection grade T cell factor (Tcf) reporter plasmid containing two full and one incomplete copy of the Tcf binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type Tcf binding sites.

Size
5.5kb
Applications
Suitable for use in TCF activity assays.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by immunoaffinity chromatography.
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lu, D., et al., Proc. Natl. Acad. Sci. USA 101: 3118-23, 2004. 2. Haq, S., et al., Proc. Natl. Acad. Sci. USA, 100: 4610-5, 2003. 3. Leung-Hagesteijn, C., et al., Embo. J. 20: 2160-70, 2001. 4. Coghlan, M. P., et al., Chem. Biol. 7: 793-803, 2000. 5. Ishitani, T., et al., Nature 399: 798-800. 1999. 6. Korinek, V., et al., Mol. Cell. Biol. 18: 1248-1256, 1998.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net