G8256X-ML550
Grade
Affinity PurifiedConjugate
MaxLight™550Specificity
Recognizes human GnRH Receptor (LH-RH Receptor).Applications
FC IF IHCEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeBSA Free
YesPreservative Free
YesNotes
Preservative Free
BSA Free
GnRH Receptor (LH-RH Receptor) (BSA & Azide Free) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH).
Applications
Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Paraffin. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control
Pituitary or normal ovary
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.