Tankyrases (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8, and its homolog TANK2), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. In addition to regulating the telomere and nuclear localization, TANKs also resides at the other subcellular compartments such as nuclear envelope, specifically on cytoplasmic fibers of nuclear pore complexes. TANK1 is targeted to pericentriolar domain of centrosomes during mitosis. After mitosis, TANK1 associates with the golgi as a peripheral membrane protein and implicated in targeting of GLUT4 vesicles. In the absence of insulin, GLUT4 vesicles reside in the Golgi and cytoplasm. Upon stimulation by insulin, GLUT4 vesicles undergo exocytosis and translocate to the plasma membrane. GLUT4 vesicles are characterized by the presence of glucose transporter 4 (GLUT4) and IRAP (Insulin regulated aminopeptidase). The exocytosis of GLUT4 vesicles is therefore plays an important role in uptake of glucose through Glut4 and the degradation of various vascoactive hormones by IRAP. TANKs have been colocalized in GLUT4 vesicles and shown to bind IRAP via the ANK-repeats.

TANK2 has also been shown to reside in the low-density microsomes and associate with the adapter protein, Grb14. The N-terminal 110-aa of Grb14 and 10-19 ANK-repeats of TANK2 mediate this interaction. GRB14 (mouse/rat 538 aa, human 540 aa, chromosome 2) is a member of GRB7 (Growth receptor bound proteins) family that includes Grb7, and Grb10. Grb family is characterized by the presence of a conserved, short non-catalytic SH2 domain (Src homology region 2) that binds to peptide sequences containing phopshotyrosine. Many intracellular targets of receptor tyrosine kinases contain 1 or more SH2 domains. GRB14 is widely distributed in many tissues with an abundance in testis, ovary, heart, liver, skeletal muscle, kidney, and pancreas. TANKs may play a role vesicle trafficking and in cytoplasmic signal transduction pathways via the Grg14.

Rat synthetic peptide

Highly purified

As reported

Supplied as a liquid in PBS, pH 7.2

The GRB14 control peptide sequence is 100% conserved in mouse and 87% in human Grb14

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.