Hepcidin, Human, 20aa, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Hepcidin (Hepc , hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues in humans and 25aa in mouse. In humans, 20aa and 25aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. Biological or antimicrobial activities of this HEPC peptide has not been studied.
The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS).
Cysteines were air-oxidized assuming that they will assume natural cys-cys bridging. Order of Cys-Cys bridges is unknown.
Amino Acid Sequence (linear)
Human 20aa: ICIFC CGCCH RSKCG MCCKT
Applications
Suitable for use in ELISA, Antibody Blocking, Positive Control for various antibodies to HEPC available from United States. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Antibody Blocking: 5-10ug control peptide per 1ug of IgG or 1ul of antiserum Optimal dilutions to be determined by researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Human synthetic peptide
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
