HMG-CoA Reductase, Control Peptide, Human (3-hydroxy-3-methylglutaryl-coenzyme A reductase)
AcCKDNPGENARQLAR- NH2
Synthetic (linear) peptide (AcCKDNPGENARQLAR- NH2) corresponding to aa827-840 of human HMG-CoA Reductase with an N-terminal cysteine added for conjugation. The peptide has the amino terminus blocked with an acetyl group and the carboxyl terminus blocked with an amide group.
The HMG-CoA reductase pathway, also known as MVA pathway or mevalonate-dependent (MAD) route, is an important cellular metabolic pathway present in virtually all organisms. It forms hydrophobic molecules for tasks as diverse as cell membrane maintenance, hormones, protein anchoring and N-glycosylation. Regulation and feedback: Several key enzymes can be activated through DNA transcriptional regulation on activation of SREBP (Sterol Regulatory Element Binding Protein 1 and 2). This intracellular sensor detects low cholesterol levels and stimulates endogenous production by the HMG-CoA reductase pathway, as well as increasing lipoprotein uptake by up-regulating the LDL receptor. Regulation of this pathway is also achieved by controlling the rate of translation of the mRNA, degradation of reductase and phosphorylation.
Theoretical Molecular Weight (M+H+)
1613.8 D
Applications
Suitable for use in Western Blot. Other applications not tested.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
