Interferon gamma, Recombinant, Mouse (IFNg)
Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. Recombinant murine IFNg is a 15.6kD protein containing 134 amino acid residues.
Biological Activity
Determined by its ability to inhibit the proliferation of mouse WEHI-279 cells. ED50 is ≤0.2ng/ml, corresponding to a specific activity of ≥5 x 10e6units/mg.
Amino Acid Sequence
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA, HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Concentration
~1mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 25mM sodium chloride. Reconstitute in ddH2O to 1mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
