IgG F(ab')2, Rabbit (Biotin)
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG F(ab')2 molecule
Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution: Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Concentration
~1mg/ml (UV 280)
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
