Technical Data

I8429-27N
Grade
Affinity Purified
Applications
E FC
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Interleukin 7 Receptor alpha, Mouse IgG2a Fc Fusion Protein (Interleukin-7 Receptor Subunit alpha, IL-7 Receptor Subunit alpha, IL-7R Subunit alpha, IL-7R-alpha, IL7RA, IL-7RA, IL7R, CD127, CDw127, ILRA)

CD127 (IL-7Ra) is the specific receptor component for the cytokine Interleukin -7 (IL-7). It is found on a wide variety of hematopoietic cell types including B cell precursors, and the majority of T cells. Its expression levels are decreased on T cells following activation. CD127 can dimerize with CD132 (IL-2R delta) to form a high affinity IL-7 receptor (1). CD127 engagement is necessary for T cell development in humans (2).

Molecular Structure: A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker, the mature extracellular domain of human CD127 (IL-7Ra) and murine IgG2a Fc + hinge regions (464 aa total). The molecule is dimeric with a predicted monomeric non glycosylated molecular weight of 52.8kD.
Applications
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity
Purified by Protein A affinity chromatography.
Concentration
~0.5mg/ml
Form
Supplied as liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Noguchi, M. et al., 1993, Science 262:1877, 2. Pribyl, J.A. and T.W. LeBien, 1996, Proc. Natl. Acad. Sci. 93:10348, 3. Goodwin RG, Friend D, Ziegler SF, et al. Cell. 1990; 60:941.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net