Technical Data

K1893-05
Grade
Purified
Accession Number
NP_112457
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
Klotho beta, Recombinant, Mouse (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

Klotho b, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue.1 Like Klotho, Klotho b facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex.2 The Klotho b mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-a hydroxylase (Cyp7a1) and sterol 12-a hydroxylase (Cyp8b1).2-4 Klotho b is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake.2-3, 5 The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in b-glycosidases, it was initially unclear whether they were functional enzymes.1, 6 However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho b is also possible.7 The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho b, respectively. The low identity with rat reflects aa discordance within rodent ECD.

Mouse myeloma cell line, NS0-derived recombinant protein corresponding to Phe53-Leu995 from mouse Klotho beta, with a C-terminal 10-His tag
Molecular Mass
~110.2kD. As a result of glycosylation, the recombinant mouse Klotho Beta migrates as an approximately 140kD protein in SDS-PAGE under reducing conditions.
Biological Activity
Measured by its ability to stimulate proliferation of NIH 3T3 cells. The ED50 for this effect is typically 0.75-3ug/ml in the presence of 2ug/ml of rhFGF-21.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, NS0 cells
Concentration
As reported
Form
Supplied as a liquid in PBS, 0.1 mM EDTA, 50% glycerol, pH 6.8.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Mian, I. S., 1998, Blood Cells Mol. Dis. 24:83 - 100|2. Ito, S. et al., 2005, J. Clin. Invest. 115:2202 - 2208|3. Kurosu, H. et al., 2007, J. Biol. Chem. 282:26687.|4. Lin, B. C. et al., 2007, J. Biol. Chem. 282:27277.|5. Ogawa, Y. et al., 2007, Proc. Natl. Acad. Sci USA 104:7432|6. Chang, Q. et al., 2005, Science 310: 490 - 493|7. Goetz, R. et al., 2007, Mol. Cell. Biol. 27:3417
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net