Technical Data

L1011-14
Grade
Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Lactate Dehydrogenase 4, Human

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types-M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

Lactate Dehydrogenase Isoenzyme 4 (LDH4) from human liver.
Activity
>110U/ml
Protein
~0.5mg Protein/ml (Commassie)
Specific Activity
~35Units/ml
Contaminants
CK: ≤1%
Unit Definition
One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Human liver
Form
Supplied as a suspension in 3.1M ammonium sulfate, 50mM Tris-chloride, 1mM DTT,1mM EDTA , pH 8.3
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net