Lactate oxidase (LOD) is a member of a family of FMN (flavin mononucleotide)-dependent alpha hydroxy acid oxidizing enzymes and catalyzes the oxidation of L-lactate to pyruvate with reduction of O2 to H2O2. LOD is widely employed in biosensors to measure lactate concentration.
Source
Aerococcus viridans
Appearance
Supplied as a yellow lyophilized powder.
Activity
≥20 units/mg solid
Specific Activity
≥50 units/mg protein
Unit Definition
One unit is defined as amount of enzyme which generates 1umole of hydrogen peroxide per minute at pH 6.5 at 37°C under standard assay conditions.
Contaminating Enzymes
Pyruvate Oxidase: ≤0.00004% Glucose Oxidase: ≤0.0002% Uricase: ≤0.000006% Cholesterol Oxidase: ≤0.001%
Enzyme Commission (EC) Number
1.13.12.4 ( BRENDA IUBMB )
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Source
Aerococcus viridans
Form
Yellow lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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