Loading Dye Buffer, Tri Color, 6X
Supplied with DNA Markers
Loading dye for DNA markers and samples on agarose or polyacrylamide gels. It contains three dyes for easy visual tracking. he optimized solution contains three dyes: bromophenol blue, xylene cyanol FF and orange G for visual three-color tracking of DNA migration during electrophoresis. The presence of glycerol in the solution ensures that the same sample forms a layer at the bottom of the well; the EDTA included in the solution binds divalent metal ions and inhibits metal dependent nucleases.
Recommended Dilutions
Add 1:5 of L3350-01 to DNA sample; mix 1 volume of dye with 5 volumes of DNA sample. In 1% agarose gels bromophenol blue comigrates with ~300bp fragment, xylene cyanol FF - with ~4000bp fragment and Orange G co-migrates with smallest fragments, i.e. ~50 bp.
Storage and Stability
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 0.15% orange G, 60mM EDTA, 60% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
