Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, macrophages and endothelial cells. This colonly stimulating factor is involved in both the development and function of monocytes/macropahges. Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of 158 amino acid residues.
Amino Acid Seqeunce
MEEVSEYCS HMIGSGHLQ SLQRLIDSQ METSCQITF EFVDQEQLK DPVCYLKKA FLLVQDIME DTMRFRDNT PNAIAIVQL QELSLRLKS CFTKDYEEH DKACVRTFY ETPLQLLEK VKNVFNETK NLLDKDWNI FSKNCNNSF AECSSQGHE RQSEGS
Storage and Stability
Human M-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤1ng/ml, corresponding to a specific activity of ≥1x10e6 units/mg. . Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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