Technical Data

M1203-02R
Grade
Highly Purified
Molecular Weight
14
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
BSA Free
Yes
Notes
BSA Free
Monocyte Chemotactic Protein 1, Recombinant, Rat (MCP-1)
Monocyte Chemotactic Protein-1, MCAF, CCL2

The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species.--Recombinant Rat MCP-1 (CCL2) is a 14kD protein containing 125 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines.

Recombinant corresponding to rat Monocyte Chemotactic Protein 1 expressed in E. coli.
Molecular Weight
~14kD
Amino Acid Sequence
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN
Biological Activity
Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Yoshimura, T., M. Takeya, and K. Takahashi (1991) Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem. Biophys. Res. Commun. 174:504-509. 2. Wareen, J.S., M. Jones, and C. Flory (1993) Analysis of monocyte chemoattractant protein 1-mediated lung injury using rat lung organ cultures. Am. J. Pathol. 143:894-906. 3. Zhang Y., C.A. Ernst, and B.J. Rollins (1996) MCP-1: Structure/activity analysis. Methods 10:93-103. 4. Porreca, E., C. Di Febbo, M. Reale, M. Castellani, G. Baccante, R. Barbacane, P. Conti, F. Cuccurullo, and A. Poggi (1997) Monocyte chemotactic protein 1 (MCP-1) is a mitogen for cultured rat vascular smooth muscle cells. J. Vasc. Res. 34:58-65. 5. Yamashiro, S., M. Takeya, J. Kuratsu, Y. Ushio, K. Takahashi, and T. Yoshimura (1998) Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin. Int. Allergy Immunol. 115:15-23. 6. Turler, A., N. Schward, E. Turler, J. Kalff, and A. Bauer (2002) MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat. Am. J. Physiol. Gastrointest Liver Physiol. 282:145-155. 7. Czermak, B.J., V. Sarma, N.M. Bless, H. Schmal, H.P. Freidl, and P.A. Ward (1999) In vitro and in vivo dependency of chemokine generation on C5a and TNF-a. J. Immunol. 162:2321-2325.
USBio References
No references available
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