Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.

Suitable for use in Western Blot using the polyclonal anti-nitrotyrosine. Not sutiable for use with monoclonal anti-nitrotyrosine.

Western Blot: 10ul per lane. Optimal dilutions to be determined by researcher.

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Highly Purified

Not determined

Supplied in SDS-PAGE sample buffer, reduced.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.