Pepsin, 10,000+ U/mg (Porcine), NF/USP

P3270-01

CAS Number

9001-75-6

Grade

Reagent Grade

Molecular Weight

EU Commodity Code

38220090

Shipping Temp

Storage Temp

-20°C

Pepsin, 10,000+ U/mg (Porcine), NF/USP

EC=3.4.23.1; Porcine stomach

Pepsin, an acidic protease, is the principal proteolytic enzyme of vertebrate gastric juice. Its inactive precursor, pepsinogen, is produced in stomach mucosa. Pepsin has a broad range of substrates and demonstrates an esterase activity. There are several minor pepsins designated “B”, “C”, and “D”; the major component is “A” to which the following data applies. They are chromatographically separable.

Conversion of pepsinogen to pepsin, which involves releasing a peptide, may be an autocatalytic process involving self-cleavage by the zymogen. Peptides released on activation have pepsin inhibitory activity.

CAS No

9001-75-6

Molecular Weight

~35kD

Source

Porcine stomach

Appearance

White to slight yellow, powder

Identification

Conforms

Loss on Drying

≤5%

Protease Activity

≥10,000U/mg [The activity of pepsin (10,000U/mg) is the same as strength (1:10,000NF).]

Solubility

Soluble in Water. Mostly insoluble in alcohol

Total Bacteria

≤10,000cfu/g

Yeast and Mold

≤100cfu/g

Bile-Tolerant Gram-Negative Bacteria

≤100cfu/g

E. coli

Negative/g

S. aurues

Negative/g

Salmonella

Negative/g

Optimum pH

~1.0 for such substrates as casein or hemoglobin if the substrate is native.

Inhibitors

Substrate-like epoxides

Storage and Stability

Powder may be stored at -20°C Stable for 6 months after receipt.

Note

Meets or exceeds USP/NF Specifications

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References

1. Al-Janabi, et al., J. Biol. Chem., 247:4628 (1972). 2. Anderson, W., et al., Nature, 243:417 (1973). 3. Andreeva, N.: Scand. J. Clin. Lab Invest., 52, Suppl. 210:31 (1992) 4. Anson, M.: J. Gen. Physiol., 22:79 (1938). 5. Arnon, R., and Perlmann, G.: J. Biol. Chem., 238:653 (1963) 6. Auer, H., and Glick, D.: Biochem., 23:2735 (1984) 7. Baker, L.: J. Biol. Chem., 211:701 (1954) 8. Basson, M., et al., J. Surgical Res., 44:82 (1988) 9. Bayliss, R., Knowles, J., and Wybrandt, G.: Biochem. J., 113:377 (1969) 10. Blumenfeld, O., and Perlmann, G.: J. Gen. Physiol., 42:563 (1959) 11. Blumenfeld, O., and Perlmann, G.: J. Gen. Physiol., 42:553 (1959). 12. Blumenfeld, et al., J. Biol. Chem., 235:379 (1960). 13. Bovey, F., and Yanari, S.: Pepsin, in The Enzymes, Vol. IV, (Boyer, P., Lardy, H., and Myrback, K., eds.), Academic Press, NY, 63 (1960). 14. Bustin, M., and Conway-Jacobs, A.: Intramolecular Activation of Porcine Pepsinogen, J. Biol. Chem., 246:615 (1971). 15. Chen, K., and Tang, J.: Amino Acid Sequence Around the Epoxide-Reactive Residues in Pepsin, J. Biol. Chem., 247, 2566 (1972). 16. Chen, K., et al., J. Biol. Chem., 250:5068 (1975).

USBio References

No references available

Technical Data

P3270-01

CAS Number

Grade

Molecular Weight

EU Commodity Code

Shipping Temp

Storage Temp

Pepsin, 10,000+ U/mg (Porcine), NF/USP

EC=3.4.23.1; Porcine stomach

References

USBio References