Technical Data

P3374-05A
Grade
Purified
Applications
E
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
PHD2, Human, Control Peptide (PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, C1orf12, chromosome 1 open reading frame 12)

Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. PHD2 (human 426 aa, chromosome 1q42-q43, also known as Egl nine homolog 1, EGLN1, (Hypoxia-inducible factor prolyl hydroxylase 2) (HIF-prolyl hydroxylase 2) (HIF-PH2) (HPH-2) (Prolyl hydroxylase domain-containing protein 2) PHD2 (SM-20) (PNAS-118 / PNAS-137) is widely expressed. Alternatively spliced isoforms 2 is missing 337-358 aa. It is activated by hypoxia in some cells and tissues.

Source
16aa peptide of Human PHD2/EGLN1 (1)
Location
~C-terminal
Species Sequence Homology
Chicken-87%; Mouse, rat-68%.
Applications
Suitable for use in ELISA as a control peptide for P3374-05. Other applications not tested.
Recommended Dilution
ELISA: Use 50-100 ng of control peptide/well Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Human synthetic peptide
Purity
Purified
Concentration
~1mg/ml
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Dupuy D et al (2000) Genomincs 69, 348-354; Taylor MS et al (2001) Gene 275, 125-132; Semenza GL et al (2001) Cell, 107, 1-3; Epstein ACR et al (2001) Cell 107, 43-54; Ivan M et al (2002) PNAS 99, 13459-13464; Cioffi CL et al BBRC 303, 947-953|
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net