Phosphodiesterase 4A1A, Recombinant, Human, GST-Tag (PDE4A1A)
Human PDE4A1A (GenBank Accession No. U97584), full length protein with N-terminal GST tag (MW = 100kD), expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity
Lot Specific. Sample Lot Value: 4,176 pmol/min/ug.
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay Conditions: 10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, and 0.2ng/ul PDE4A1A at 37°C for 20 min. Quantified by 5′-nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT and 20% glycerol
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
