Technical Data

P4256-29
Grade
Highly Purified
Accession Number
NM_000602
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C
Plasminogen Activator Inhibitor Type 1, Human, Recombinant (PAI 1)

The cardiovascular risk factor plasminogen activator inhibitor type 1 (PAI 1) has been associated with abdominal obesity, hypertension, hypertriglyceridemia, hyperinsulinemia, glucose intolerance, and type II diabetes, conditions known to be linked with insulin resistance. Plasminogen activator inhibitor 1 (PAI 1) is a member of the serine protease inhibitor (serpin) superfamily and a central regulatory protein in the blood coagulation system. PAI-1 is unique among serpins in exhibiting distinct active and inactive (latent) conformations in vivo. Human PAI 1 is a single-chain glycoprotein with a molecular weight of 43kD. This inhibitor acts as “bait” for tissue-type and urokinase-type plasminogen activators (tPA and uPA) and protein C. Its rapid interaction with tPA may function as a major control point in the regulation of fibrinolysis. The highly mobile reactive-center loop (RCL) is thought to account for both the rapid inhibiton of plasminogen activators, and the rapid and spontaneous transition of the unstable, active form of PAI 1 into the stable, inactive conformation. The inactive form can be partially reactivated by denaturants such as urea, guanidine hydrochloride or SDS. High concentrations of PAI 1 have been associated with human thromboembolic disease. PAI 1 activity may limit the extent of tumor metastasis, since uPA activity is a major contributory factor promoting dissolution of tumor matrix and basement membrane.

The sequence corresponding to aa24 -427 of human PAI-1 (mature form) tagged with 6xHis on the carboxy-terminal was expressed in E. coli.
By uPA activity assay, the recombinant PAI-1 contains a mixture of the active and latent forms, with ≥ 45% of active form. The recombinant PAI-1 is able to bind to uPA resulting in a SDS-stable complex.
Activity
To fully activate the latent PAI-1, the PAI-1 must be incubated with Activation Buffer (2X Activation Buffer: 8 M Guanidine HCl, 40 mM Sodium Acetate, pH 5.6, 400 mM sodium chloride, 0.2% Tween 20). The recombinant PAI-1 is able to bind to uPA resulting in a SDS-stable complex.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt.
Source
Recombinant, E. coli
Purity
~98% (SDS-PAGE gel electrophoresis)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in PBS, pH 6.6, 2mM Glutathione, 0.01% Tween 80
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. P.M. Shen-nan, et al. (1992) J. Biol. Chem. 267, 7588–7595. 2. D.A. Lawrence, et al. (1994) Biochemistry 33, 3643–3648. 3. D.A. Lawrence, et al. (1994] J. Biol. Chem. 269,15223–15228. 4. M.B. Berkenpas, et al. (1995) EMBO J. 14, 2969–2977. 5. H.T Wright (1996) BioEssays 18,453–464. 6. G.C. Prendergast, et al. (1990) Mol. Cell. Biol. 10, 1265–1269. 7, R. Zeheb and T.D. Glehrter (1988) Gene 73, 459–468.
USBio References
No references available
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