Plasminogen Activator Inhibitor Type 1, Human, active wild type (PAI-1) (Biotin)
Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6).
Solubility
>2mg/ml and <5mg/ml
Second order rate constants for inhibition of
uPA = 6.3 X 10e6 M^-1s^-1 tPA = 5.7 X 10e6 M^-1s^-1
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Purity
≥98% by SDS PAGE 95 ± 5% active by uPA titration
Form
Supplied as a liquid in 0.05M sodium phosphate; 0.1M sodium chloride; 1mM EDTA; pH 6.6 Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
