Prostatic Acid Phosphatase, Recombinant, Human, His-Tag (ACPP, 5'-nucleotidase, 5'-NT, TMPase, Ecto-5'-nucleotidase, Thiamine Monophosphatase)
Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of ACPP is in the pH range of 4-6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Cellular ACPP has been shown to be a protein tyrosine phosphatase. Protein substrates include the epidermal growth factor receptor and HER-2. Cellular ACPP is considered to function as a tumor suppressor.
Recombinant corresponding to Met1-Gln379 from human ACPP, fused to 6-His-tag at C-terminal expressed in mouse myeloma cell line, NS0.
Biological Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
Specific Activity
>100,000pmol/min/ug
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, NS0 cells
Form
Supplied as a liquid in 25mM Tris, 150mM sodium chloride, pH 7.5
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
