Resistin-like Molecule, alpha, Recombinant, Mouse (RELMa)
RELMα belongs to a unique family of tissue-specific cytokines termed FIZZ (found in inflammatory zone) and RELM. The four known members of this family, resistin, RELMa, RELMb, and RELMγ, are 85-94 amino acid, secreted proteins sharing a conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. RELMa and resistin are secreted exclusively by adipocytes, while RELMb is expressed in the epithelium of the colon and small bowel. The physiological role and molecular targets of RELMa are still unknown.
Recombinant Mmouse RELMa is a 10kD monomeric protein containing 89 amino acid residues.
Amino Acid Sequence
MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder in 10mM Tris, pH 7.5, 25mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml Do not vortex. It is recommended that further dilutions be made in PBS, 0.1% BSA or HSA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.