Technical Data

R1691-10A
Grade
Purified
Applications
E WB
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Retinoic Acid Receptor gamma, Control Peptide (RAR gamma, RARg)

Hinge region peptide amino acids 213-226 of human RAR gamma [lglwdkfselatkc] (linear). Corresponding antibody R1691-10 is specific for Retinoic acid receptor, gamma isotype. Recognizes both isoforms of RAR gamma: RAR gamma-1 and RAR gamma-2. Species crossreactivity: Human and rat RAR gamma.

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition (1). While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrettís tissues compared with normal tissue (3).
Applications
Suitable for use in ELISA and Western Blotting. Other applications not tested.
Recommended Dilution
Western blot: 1:1000. TBST (20 mM Tris/HCl (pH 7.6), 137mM NaCl, 1% Tween-20) 5% nonfat milk, 0.05% sodium azide. A ~48kD band can be seen using rat brain hippocampal homogenate. The investigator should determine the optimal working dilution for a specific application.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Synthetic peptide
Purity
95+%, HPLC, Mass Spec
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Klaassen, I., et al. Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines. Int. J. Cancer. 92(5):661-665, 2001. 2. Lefebvre, B., et al. Phosphorylation of Histone H3 is functionally linked to retinoic acid receptor {beta} promoter activation. EMBO. 3(4): 335-340, 2002. 3. Lord, R.V. et al. Retinoic acid receptor-alpha messenger RNA expression is increased and retinoic acid receptor-gamma expression is decreased in Barrettís intestinal metaplasia, dysplasia, and adenocarcinoma sequence. Surgery. 129(3): 267-76, 2001.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net