SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2)
Human Sphingosine kinase 2 ( GenBank Accession No.NM_020126), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity
20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min.
Assay Buffer
50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.75ug/ml sphingosine kinase 2.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Source
Baculovirus infected Sf9 cells
Form
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol and 3mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
