Technical Data

S7973-01H
Grade
Highly Purified
Molecular Weight
18.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
Stem Cell Factor, Recombinant, Human, aa26-189 (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Source
Recombinant corresponding to aa26-189 of human Stem Cell Factor expressed in E.coli.
Amino Acid Sequence
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥ 95% by SDS-PAGE. Purified by using conventional chromatography techniques.
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zhang, Z., et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97: 7732 (2000). 2. Okada, S., et al., Nippon Rinsho 50: 1872 (1992).
USBio References
No references available
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