S7973-02
Grade
Highly PurifiedGene ID
4254Molecular Weight
18.4EU Commodity Code
30021019Shipping Temp
RTStorage Temp
-20°CStem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Recombinant protein corresponding to human Stem Cell Factor, expressed in E. coli.
Amino Acid Sequence
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2ng/ml, corresponding to a specific activity of ≥ 5x10e5 units/mg.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder from 0.02% sodium bicarbonate. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Zheng Y, Watanabe N, Igura K, Takaue Y, Exp Hematol 20 03 Dec;31(12):1237-46 2. Moon TC, Lee E, Murakami M, Min HK, Mol Cells 2003 Oct 31;16(2):154-60 3. Han J, Yan X, Zhi X, Protein Expr Purif 2003 Oct;31(2):311-7 4. [Expression of recombinant human stem cell factor in insect cells] Zhang D, Qin J, Zhu J, Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9 5. [The expression study of human stem cell factor (hSCF) in E. coli] Mao H, Zhao J, Di X, Zhongguo Yi Xue Ke Xue Yuan Xue Bao 1998 Aug;20(4):279-84 6. Zhang Z, Zhang R, Schlessinger J, Proc Natl Acad Sci U S A 2000 Jul 5;97(14):7732-7.USBio References
No references available